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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F47834 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nestin Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Nestin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate; Predicted band size : 170-220 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Nestin antibody and HepG2 lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with Nestin antibody at 1:200. Note the highly specific localization of the Nestin immunosignal to the intermediate filaments.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma stained with Nestin antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using Nestin antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
