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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP07829PU-N - Provider product page
- Provider
- OriGene
- Product name
- Nestin (NES) (1484-1500) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Nestin (NES) (1484-1500) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NES
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-Nestin Antibody - Western Blot. Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4°C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Nestin antibody staining of Formalin-Fixed Paraffin-Embedded Mouse Kidney.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Nestin antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 5 ug/ml
- Validation comment
- IHC
