LS-C814933

antibody from LSBio

Targeting: NES FLJ21841

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C814933

Provider

LSBio

Product name

NES / Nestin Antibody (Preservative Free) LS-C814933

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-NES rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: U251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa. Observed band size: 250 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

NES was immunoprecipitated using: Lane A: 0.5 mg U251MG Whole Cell Lysate. 4 uL anti-NES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa. Observed band size: 250 kDa.