600-401-417

antibody from Rockland Immunochemicals, Inc.

Targeting: NES FLJ21841

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-417

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-417, RRID:AB_2235741

Product name

Anti-Nestin (RABBIT) Antibody - 600-401-417

Antibody type

Polyclonal

Vial size

100 µl

Immunohistochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunohistochemistry with Anti-nestin antibody at 40X (left) and 20X (right) Tissue: Brain and Cerebellum (right) Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20ug/ml 1 hour @ room T Secondary antibody: Goat anti Rabbit Polymer HRP Prediluted by the manufacturer 30 min. @ room T Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Validation comment

Immunohistochemistry