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Western blot
Immunocytochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- STJA0003696 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-NES antibody [4D11] (STJA0003696)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Nestin is suitable for use in Western Blot and Immunofluorescence research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C, and avoid repeated freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. STJA0003696, red, 1:500) and anti-MAP2 antibody (cat. STJA0003676, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. STJA0003696, red, 1:500) and anti-vimentin antibody (cat. STJA0003823, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
