Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX113057 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113057, RRID:AB_11174793
- Product name
- NAK antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE GTX113057 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NAK antibody detects NAK protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAK antibody (GTX113057) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAK antibody [N2C1], Internal (GTX113057) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NAK antibody [N2C1], Internal detects NAK protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: NAK protein stained by NAK antibody [N2C1], Internal (GTX113057) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NAK antibody [N2C1], Internal detects TBK1 protein at cytoplasm on H441 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H441 xenograft. NAK antibody [N2C1], Internal (GTX113057) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NAK antibody [N2C1], Internal detects NAK protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. NAK antibody [N2C1], Internal (GTX113057) dilution: 1:500.
