NB100-56705

antibody from Novus Biologicals

Targeting: TBK1 NAK

Provider product page for NB100-56705

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [28]
Comments [0]
Validations
Western blot [4]
Immunohistochemistry [4]

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Product number: NB100-56705 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB100-56705, RRID:AB_2199766
Product name: Mouse Monoclonal TBK1 Antibody
Antibody type: Monoclonal
Description: Protein G purified.
Reactivity: Human, Mouse, Rat, Bovine, Canine
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

TBK1 protein structure - NB100-56705 shown in red.

Submitted references TRAF3IP3 negatively regulates cytosolic RNA induced anti-viral signaling by promoting TBK1 K48 ubiquitination.

Deng M, Tam JW, Wang L, Liang K, Li S, Zhang L, Guo H, Luo X, Zhang Y, Petrucelli A, Davis BK, Conti BJ, June Brickey W, Ko CC, Lei YL, Sun S, Ting JP
Nature communications 2020 May 4;11(1):2193

Anti-influenza A virus mechanism of three representative compounds from Flos Trollii via TLRs signaling pathways.

Shi D, Chen M, Liu L, Wang Q, Liu S, Wang L, Wang R
Journal of ethnopharmacology 2020 May 10;253:112634

VAMP8 Contributes to the TRIM6-Mediated Type I Interferon Antiviral Response during West Nile Virus Infection.

van Tol S, Atkins C, Bharaj P, Johnson KN, Hage A, Freiberg AN, Rajsbaum R
Journal of virology 2020 Jan 6;94(2)

NSs Protein of Sandfly Fever Sicilian Phlebovirus Counteracts Interferon (IFN) Induction by Masking the DNA-Binding Domain of IFN Regulatory Factor 3.

Wuerth JD, Habjan M, Wulle J, Superti-Furga G, Pichlmair A, Weber F
Journal of virology 2018 Dec 1;92(23)

Signalling strength determines proapoptotic functions of STING.

Gulen MF, Koch U, Haag SM, Schuler F, Apetoh L, Villunger A, Radtke F, Ablasser A
Nature communications 2017 Sep 5;8(1):427

Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) suppresses STING-mediated DNA sensing pathway through inducing mitochondrial fission.

Kwon D, Park E, Sesaki H, Kang SJ
Biochemical and biophysical research communications 2017 Nov 4;493(1):737-743

Optineurin deficiency in mice is associated with increased sensitivity to Salmonella but does not affect proinflammatory NF-κB signaling.

Slowicka K, Vereecke L, Mc Guire C, Sze M, Maelfait J, Kolpe A, Saelens X, Beyaert R, van Loo G
European journal of immunology 2016 Apr;46(4):971-80

Redox-modulating agents target NOX2-dependent IKKε oncogenic kinase expression and proliferation in human breast cancer cell lines.

Mukawera E, Chartier S, Williams V, Pagano PJ, Lapointe R, Grandvaux N
Redox biology 2015 Dec;6:9-18

Autophagy facilitates Salmonella replication in HeLa cells.

Yu HB, Croxen MA, Marchiando AM, Ferreira RB, Cadwell K, Foster LJ, Finlay BB
mBio 2014 Mar 11;5(2):e00865-14

Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid.

Conlon J, Burdette DL, Sharma S, Bhat N, Thompson M, Jiang Z, Rathinam VA, Monks B, Jin T, Xiao TS, Vogel SN, Vance RE, Fitzgerald KA
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5216-25

TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2.

Delhase M, Kim SY, Lee H, Naiki-Ito A, Chen Y, Ahn ER, Murata K, Kim SJ, Lautsch N, Kobayashi KS, Shirai T, Karin M, Nakanishi M
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 24;109(4):E177-86

IFN-β expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling.

Tamassia N, Bazzoni F, Le Moigne V, Calzetti F, Masala C, Grisendi G, Bussmeyer U, Scutera S, De Gironcoli M, Costantini C, Musso T, Cassatella MA
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1500-9

Rebamipide suppresses TLR-TBK1 signaling pathway resulting in regulating IRF3/7 and IFN-α/β reduction.

Ogasawara N, Sasaki M, Itoh Y, Tokudome K, Kondo Y, Ito Y, Tanida S, Kamiya T, Kataoka H, Joh T, Kasugai K
Journal of clinical biochemistry and nutrition 2011 Mar;48(2):154-60

Akt contributes to activation of the TRIF-dependent signaling pathways of TLRs by interacting with TANK-binding kinase 1.

Joung SM, Park ZY, Rani S, Takeuchi O, Akira S, Lee JY
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):499-507

Toll-like receptor 4 signal transduction in platelets: novel pathways.

Berthet J, Damien P, Hamzeh-Cognasse H, Pozzetto B, Garraud O, Cognasse F
British journal of haematology 2010 Oct;151(1):89-92

Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality.

Marchlik E, Thakker P, Carlson T, Jiang Z, Ryan M, Marusic S, Goutagny N, Kuang W, Askew GR, Roberts V, Benoit S, Zhou T, Ling V, Pfeifer R, Stedman N, Fitzgerald KA, Lin LL, Hall JP
Journal of leukocyte biology 2010 Dec;88(6):1171-80

Homeostatic maintenance of pathogen-containing vacuoles requires TBK1-dependent regulation of aquaporin-1.

Radtke AL, O'Riordan MX
Cellular microbiology 2008 Nov;10(11):2197-207

Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils.

Tamassia N, Le Moigne V, Rossato M, Donini M, McCartney S, Calzetti F, Colonna M, Bazzoni F, Cassatella MA
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6563-73

Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils.

Tamassia N, Le Moigne V, Rossato M, Donini M, McCartney S, Calzetti F, Colonna M, Bazzoni F, Cassatella MA
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6563-73

Glucocorticoids inhibit IRF3 phosphorylation in response to Toll-like receptor-3 and -4 by targeting TBK1 activation.

McCoy CE, Carpenter S, Pålsson-McDermott EM, Gearing LJ, O'Neill LA
The Journal of biological chemistry 2008 May 23;283(21):14277-85

Suppression of viral gene expression in bovine leukemia virus-associated B-cell malignancy: interplay of epigenetic modifications leading to chromatin with a repressive histone code.

Merimi M, Klener P, Szynal M, Cleuter Y, Kerkhofs P, Burny A, Martiat P, Van den Broeke A
Journal of virology 2007 Jun;81(11):5929-39

The MyD88-independent pathway is not mobilized in human neutrophils stimulated via TLR4.

Tamassia N, Le Moigne V, Calzetti F, Donini M, Gasperini S, Ear T, Cloutier A, Martinez FO, Fabbri M, Locati M, Mantovani A, McDonald PP, Cassatella MA
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 1;178(11):7344-56

The MyD88-independent pathway is not mobilized in human neutrophils stimulated via TLR4.

Regulation of IkappaB kinase epsilon expression by the androgen receptor and the nuclear factor-kappaB transcription factor in prostate cancer.

Péant B, Diallo JS, Lessard L, Delvoye N, Le Page C, Saad F, Mes-Masson AM
Molecular cancer research : MCR 2007 Jan;5(1):87-94

Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells.

Aksoy E, Albarani V, Nguyen M, Laes JF, Ruelle JL, De Wit D, Willems F, Goldman M, Goriely S
Blood 2007 Apr 1;109(7):2887-93

IKK-i signals through IRF3 and NFkappaB to mediate the production of inflammatory cytokines.

Sankar S, Chan H, Romanow WJ, Li J, Bates RJ
Cellular signalling 2006 Jul;18(7):982-93

Gene expression and antiviral activity of alpha/beta interferons and interleukin-29 in virus-infected human myeloid dendritic cells.

Osterlund P, Veckman V, Sirén J, Klucher KM, Hiscott J, Matikainen S, Julkunen I
Journal of virology 2005 Aug;79(15):9608-17

IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.

Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT, Coyle AJ, Liao SM, Maniatis T
Nature immunology 2003 May;4(5):491-6

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE))

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.