Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A301-951A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDZ-RhoGEF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PDZ-RhoGEF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PDZ-RhoGEF (Cat. No. A301-951A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PDZ-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDZ-RhoGEF antibody A301-951A used for IP at 3 µg/mg lysate. PDZ-RhoGEF was also immunoprecipitated by rabbit anti-PDZ-RhoGEF antibody A301-952A, which recognizes a downstream epitope. For blotting immunoprecipitated PDZ-RhoGEF, A301-952A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.