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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81102 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81102, RRID:AB_11170361
- Product name
- PXR antibody, Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Relationship between CYP3A29 and pregnane X receptor in landrace pigs: Pig CYP3A29 has a similar mechanism of regulation to human CYP3A4.
He Y, Zhou X, Li X, Jin X, Wang X, Pan X, Bi D
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2018 Dec;214:9-16
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2018 Dec;214:9-16
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of NR1I2 Antibody (Center) (GTX81102) in HL-60 cell line lysates (35ug/lane). NR1I2 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NR1I2 Antibody (Center)(GTX81102) with HepG2 cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NR1I2 Antibody (Center)(GTX81102), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NR1I2 Antibody (Center) (GTX81102) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
