616902

antibody from BioLegend

Targeting: NR1I2 BXR, ONR1, PAR2, PXR, SXR

Western blot (Data presented on provider website)

Antibody Data

Product number

616902

Provider

BioLegend

Proper citation

BioLegend Cat#616902, RRID:AB_2155075

Product name

Purified anti-PXR

Antibody type

Polyclonal

Antigen

Recombinant (partial) , N-terminal

Description

PXR (also known as orphan nuclear receptor PXR, pregnane X receptor, orphan nuclear receptor PAR1, and steroid and xenobiotic receptor (SXR)) is a member of the nuclear hormone receptor family, NR1 subfamily containing a zinc finger domain. Alternatively spliced variants of this receptor have been reported. This nuclear protein has a molecular weight of 50 kD and is highly expressed in liver, colon, and small intestine. PXR is an orphan receptor whose natural ligand is probably pregnane. PXR binds to the promoter response element in cytochrome p450 gene monooxygenase CYP3A4 and activates expression in response to variety of endobiotics and xenobiotics. PXR is activated by naturally occurring steroids such as pregnenolone and progesterone and can also be activated by dexamethasone and rifampicin. PXR forms a heterodimer with RXR. The Poly6169 antibody recognizes human and mouse PXR and has been shown to be useful for Western blotting.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

200 µl

Storage

Upon receipt, store frozen at -20° C.

References

Submitted references

Restoring blood-brain barrier P-glycoprotein reduces brain amyloid-beta in a mouse model of Alzheimer's disease.

Hartz AM, Miller DS, Bauer B
Molecular pharmacology 2010 May;77(5):715-23

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Expanding the roles for pregnane X receptor in cancer: proliferation and drug resistance in ovarian cancer.

Gupta D, Venkatesh M, Wang H, Kim S, Sinz M, Goldberg GL, Whitney K, Longley C, Mani S
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Sep 1;14(17):5332-40

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The phytoestrogen coumestrol is a naturally occurring antagonist of the human pregnane X receptor.

Wang H, Li H, Moore LB, Johnson MD, Maglich JM, Goodwin B, Ittoop OR, Wisely B, Creech K, Parks DJ, Collins JL, Willson TM, Kalpana GV, Venkatesh M, Xie W, Cho SY, Roboz J, Redinbo M, Moore JT, Mani S
Molecular endocrinology (Baltimore, Md.) 2008 Apr;22(4):838-57

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Human pregnane X receptor and resistance to chemotherapy in prostate cancer.

Chen Y, Tang Y, Wang MT, Zeng S, Nie D
Cancer research 2007 Nov 1;67(21):10361-7