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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- CD0330TR - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3e Monoclonal Antibody (UCHT1), Pacific Orange™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis. Each lot is tested by flow cytometry using human peripheral blood leukocytes (PBL).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UCHT1
- Vial size
- 25 Tests
- Storage
- 4° C, store in dark
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Antigen Specificity: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR/CD3 complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by developing thymocytes and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 results in T cell proliferation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
