NBP2-76399

antibody from Novus Biologicals

Targeting: CD247 CD3H, CD3Q, CD3Z

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-76399

Provider

Novus Biologicals

Product name

Rabbit Monoclonal CD3 zeta Antibody

Antibody type

Monoclonal

Description

Antigen Affinity-purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 2 - 8 C 1 year from date of receipt

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of Jurkat, MOLT-4, K-562, SR, HeLa, and HEK293T lysate. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2] (NBP2-76399). Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Metastatic lymph node from lung carcinoma by IHC. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2] (NBP2-76399). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2] (NBP2-76399). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.