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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA328701 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-alpha1B-Adrenoceptor (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-alpha1B-Adrenoceptor (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ADRA1B
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of a1B-adrenoreceptor in lysates of rat brain (lanes 1and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6): 1,2,5. Anti-a1B-adrenoreceptor (extracellular) antibody, (1:200). 3,4,6. Anti-a1B-adrenoreceptor (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of a1B-adrenoreceptor in GH3 cells. Immunocytochemical staining of GH3 cells with Anti-a1B-adrenoreceptor (extracellular) antibody, followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the DNA dye Hoechst 33342 (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Anti-a1B-Adrenoceptor in rat cerebellum.Immunohistochemical staining of frozen section of rat cerebellum using Anti-Anti-a1B-Adrenoceptor (extracellular) antibody, (1:100). a1B-Adrenoceptor (red) is detected in Purkinje cells. Hoechst 33342 was used as the counterstain.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect flow cytometry analysis of GH3 living cells: black line, Unstained cells. green line, Cells + Anti-a1B-adrenoreceptor (extracellular) antibody, (10 ug/5x105 cells).
- Validation comment
- FC
