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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX104227 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104227, RRID:AB_1950598
- Product name
- IRAK3 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX104227 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX104227 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRAK3 antibody [N1C1] (GTX104227) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IRAK3(GTX104227) antibody at 1:200 dilution.
