PAB10252

antibody from Abnova Corporation

Targeting: IRAK3 IRAK-M

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10252

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10252, RRID:AB_1676576

Product name

IRAK3 polyclonal antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to C-terminus of human IRAK3.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 μg

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family.

Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z
The Journal of biological chemistry 1999 Jul 2;274(27):19403-10

---

IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.

Muzio M, Ni J, Feng P, Dixit VM
Science (New York, N.Y.) 1997 Nov 28;278(5343):1612-5

---

IRAK: a kinase associated with the interleukin-1 receptor.

Cao Z, Henzel WJ, Gao X
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using IRAK3 polyclonal antibody (Cat # PAB10252) shows detection of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates. The membrane was probed with the primary antibody diluted to at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry using IRAK3 polyclonal antibody (Cat # PAB10252) shows staining of IRAK3 in rat liver.Paraffin embedded formalin fixed tissue was reacted with the primary antibody diluted to 1 : 500.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)