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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00011213-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011213-M02, RRID:AB_539913
- Product name
- Mouse Monoclonal IRAK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. IRAK3 - interleukin-1 receptor-associated kinase 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRAK3 Antibody (1F6) [H00011213-M02] - IRAK3 monoclonal antibody (M02), clone 1F6 Western Blot analysis of IRAK3 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRAK3 Antibody (1F6) [H00011213-M02] - Analysis of IRAK3 over-expressed 293 cell line, cotransfected with IRAK3 Validated Chimera RNAi ( Cat # H00011213-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK3 monoclonal antibody (M02) clone 1F6 (Cat # H00011213-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRAK3 Antibody (1F6) [H00011213-M02] - Analysis of IRAK3 expression in transfected 293T cell line by IRAK3 monoclonal antibody (M02), clone 1F6.Lane 1: IRAK3 transfected lysate(67.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: IRAK3 Antibody (1F6) [H00011213-M02] - Detection limit for recombinant GST tagged IRAK3 is approximately 0.3ng/ml as a capture antibody.
