Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB0066 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0066, RRID:AB_1017007
- Product name
- TLR2 monoclonal antibody, clone 1030A5.138
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant TLR2.
- Isotype
- IgG
- Antibody clone number
- 1030A5.138
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Activation of toll-like receptor-2 by endogenous matrix metalloproteinase-2 modulates dendritic-cell-mediated inflammatory responses.
Tissue expression of Toll-like receptors 2 and 4 in sporadic human colorectal cancer.
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
Microarray analysis reveals induction of lipoprotein genes in mucoid Pseudomonas aeruginosa: implications for inflammation in cystic fibrosis.
Soluble forms of Toll-like receptor (TLR)2 capable of modulating TLR2 signaling are present in human plasma and breast milk.
Glucocorticoids synergistically enhance nontypeable Haemophilus influenzae-induced Toll-like receptor 2 expression via a negative cross-talk with p38 MAP kinase.
Godefroy E, Gallois A, Idoyaga J, Merad M, Tung N, Monu N, Saenger Y, Fu Y, Ravindran R, Pulendran B, Jotereau F, Trombetta S, Bhardwaj N
Cell reports 2014 Dec 11;9(5):1856-70
Cell reports 2014 Dec 11;9(5):1856-70
Tissue expression of Toll-like receptors 2 and 4 in sporadic human colorectal cancer.
Nihon-Yanagi Y, Terai K, Murano T, Matsumoto T, Okazumi S
Cancer immunology, immunotherapy : CII 2012 Jan;61(1):71-7
Cancer immunology, immunotherapy : CII 2012 Jan;61(1):71-7
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Microarray analysis reveals induction of lipoprotein genes in mucoid Pseudomonas aeruginosa: implications for inflammation in cystic fibrosis.
Firoved AM, Ornatowski W, Deretic V
Infection and immunity 2004 Sep;72(9):5012-8
Infection and immunity 2004 Sep;72(9):5012-8
Soluble forms of Toll-like receptor (TLR)2 capable of modulating TLR2 signaling are present in human plasma and breast milk.
LeBouder E, Rey-Nores JE, Rushmere NK, Grigorov M, Lawn SD, Affolter M, Griffin GE, Ferrara P, Schiffrin EJ, Morgan BP, Labéta MO
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 15;171(12):6680-9
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 15;171(12):6680-9
Glucocorticoids synergistically enhance nontypeable Haemophilus influenzae-induced Toll-like receptor 2 expression via a negative cross-talk with p38 MAP kinase.
Shuto T, Imasato A, Jono H, Sakai A, Xu H, Watanabe T, Rixter DD, Kai H, Andalibi A, Linthicum F, Guan YL, Han J, Cato AC, Lim DJ, Akira S, Li JD
The Journal of biological chemistry 2002 May 10;277(19):17263-70
The Journal of biological chemistry 2002 May 10;277(19):17263-70
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TLR2 in 30 ugs of Ramos whole cell lysate. Using TLR2 monoclonal antibody, clone 1030A5.138 (Cat # MAB0066) at 1 : 500.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IHC analysis of TLR2 in paraffin-embedded human spleen tissue. Using TLR2 monoclonal antibody, clone 1030A5.138 (Cat # MAB0066) at 1 : 100 (left) and isotype control (right) .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR2 in 106 THP-1 cells. Using 5 uL of TLR2 monoclonal antibody, clone 1030A5.138 (Cat # MAB0066). The shaded histogram represents ThP-1 cells with no antibody present; Green represents isotype control ; red represents TLR2 antibody.
- Validation comment
- Flow Cytometry
