ALX-804-323PF-C050

antibody from Enzo Life Sciences

Targeting: TLR2 CD282, TIL4

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Data presented on provider website)

Functional assay (Recommended by provider)

Antibody Data

Product number

ALX-804-323PF-C050

Provider

Enzo Life Sciences

Product name

Toll-like receptor 2 (human) monoclonal antibody (TL2.1) (preservative free)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

TL2.1

Vial size

50 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies.

Satta N, Dunoyer-Geindre S, Reber G, Fish RJ, Boehlen F, Kruithof EK, de Moerloose P
Blood 2007 Feb 15;109(4):1507-14

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Divergent trophoblast responses to bacterial products mediated by TLRs.

Abrahams VM, Bole-Aldo P, Kim YM, Straszewski-Chavez SL, Chaiworapongsa T, Romero R, Mor G
Journal of immunology (Baltimore, Md. : 1950) 2004 Oct 1;173(7):4286-96

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Haemophilus influenzae porin induces Toll-like receptor 2-mediated cytokine production in human monocytes and mouse macrophages.

Galdiero M, Galdiero M, Finamore E, Rossano F, Gambuzza M, Catania MR, Teti G, Midiri A, Mancuso G
Infection and immunity 2004 Feb;72(2):1204-9

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Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells.

Medvedev AE, Lentschat A, Wahl LM, Golenbock DT, Vogel SN
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5209-16

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Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses.

Sabroe I, Jones EC, Usher LR, Whyte MK, Dower SK
Journal of immunology (Baltimore, Md. : 1950) 2002 May 1;168(9):4701-10

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Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction.

Latz E, Visintin A, Lien E, Fitzgerald KA, Monks BG, Kurt-Jones EA, Golenbock DT, Espevik T
The Journal of biological chemistry 2002 Dec 6;277(49):47834-43

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A novel host-parasite lipid cross-talk. Schistosomal lyso-phosphatidylserine activates toll-like receptor 2 and affects immune polarization.

van der Kleij D, Latz E, Brouwers JF, Kruize YC, Schmitz M, Kurt-Jones EA, Espevik T, de Jong EC, Kapsenberg ML, Golenbock DT, Tielens AG, Yazdanbakhsh M
The Journal of biological chemistry 2002 Dec 13;277(50):48122-9

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Two lipoproteins extracted from Escherichia coli K-12 LCD25 lipopolysaccharide are the major components responsible for Toll-like receptor 2-mediated signaling.

Lee HK, Lee J, Tobias PS
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 15;168(8):4012-7

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Differential expression of Toll-like receptor 2 in human cells.

Flo TH, Halaas O, Torp S, Ryan L, Lien E, Dybdahl B, Sundan A, Espevik T
Journal of leukocyte biology 2001 Mar;69(3):474-81

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A novel synthetic acyclic lipid A-like agonist activates cells via the lipopolysaccharide/toll-like receptor 4 signaling pathway.

Lien E, Chow JC, Hawkins LD, McGuinness PD, Miyake K, Espevik T, Gusovsky F, Golenbock DT
The Journal of biological chemistry 2001 Jan 19;276(3):1873-80

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Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation.

Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M
Journal of immunology (Baltimore, Md. : 1950) 2001 Feb 1;166(3):2018-24

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Monocytic cell activation by Nonendotoxic glycoprotein from Prevotella intermedia ATCC 25611 is mediated by toll-like receptor 2.

Sugawara S, Yang S, Iki K, Hatakeyama J, Tamai R, Takeuchi O, Akashi S, Espevik T, Akira S, Takada H
Infection and immunity 2001 Aug;69(8):4951-7

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Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae.

Wang JE, Warris A, Ellingsen EA, Jørgensen PF, Flo TH, Espevik T, Solberg R, Verweij PE, Aasen AO
Infection and immunity 2001 Apr;69(4):2402-6

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Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide.

Flo TH, Halaas O, Lien E, Ryan L, Teti G, Golenbock DT, Sundan A, Espevik T
Journal of immunology (Baltimore, Md. : 1950) 2000 Feb 15;164(4):2064-9

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Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products.

Lien E, Sellati TJ, Yoshimura A, Flo TH, Rawadi G, Finberg RW, Carroll JD, Espevik T, Ingalls RR, Radolf JD, Golenbock DT
The Journal of biological chemistry 1999 Nov 19;274(47):33419-25

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometryÊusing MAb to Toll-like Receptor 2 (human) (TL2.1) (Prod. No. ALX-804-323) forÊintracellular staining (A) and extracellular staining (B) of human monocytesÊby flow cytometry. Histogram on the leftÊrepresents a control staining using an isotype-matched control antibody.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometryÊusing MAb to Toll-like Receptor 2 (human) (TL2.1) (Prod. No. ALX-804-323) to stain CHO cells that stably express human TLR2 by flow cytometry. Histogram on the leftÊrepresents a control staining using an isotype-matched control antibody.