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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- AF2616 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TLR2 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant mouse TLR2 and less than 0.3% cross-reactivity with recombinant human (rh) TLR3 and rhTLR4/MD-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O60603- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Toll-like receptor 2 is increased in neurons in Parkinson's disease brain and may contribute to alpha-synuclein pathology.
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Maternal high-fat diet disturbs uteroplacental hemodynamics and increases the frequency of stillbirth in a nonhuman primate model of excess nutrition.
Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo.
Dzamko N, Gysbers A, Perera G, Bahar A, Shankar A, Gao J, Fu Y, Halliday GM
Acta neuropathologica 2017 Feb;133(2):303-319
Acta neuropathologica 2017 Feb;133(2):303-319
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Wilhelmsen K, Mesa KR, Lucero J, Xu F, Hellman J
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
Maternal high-fat diet disturbs uteroplacental hemodynamics and increases the frequency of stillbirth in a nonhuman primate model of excess nutrition.
Frias AE, Morgan TK, Evans AE, Rasanen J, Oh KY, Thornburg KL, Grove KL
Endocrinology 2011 Jun;152(6):2456-64
Endocrinology 2011 Jun;152(6):2456-64
Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo.
Shin HS, Xu F, Bagchi A, Herrup E, Prakash A, Valentine C, Kulkarni H, Wilhelmsen K, Warren S, Hellman J
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):1119-30
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):1119-30
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TLR2 by Western Blot. Western blot shows lysate of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
