PA1020
antibody from Boster Biological Technology
Targeting: CD40LG
CD154, CD40L, gp39, hCD40L, HIGM1, IMD3, TNFSF5, TRAP
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1020 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD40L/CD40LG Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CD40L/CD40LG detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CD40L/CD40LG information: Molecular Weight: 29274 MW; Subcellular Localization: Cell membrane; Single-pass type II membrane protein; Tissue Specificity: Specifically expressed on activated CD4+ T- lymphocytes.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of sCD40L using anti- sCD40L antibody (PA1020). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: HELA Whole Cell Lysate, Lane 3: JURKAT Whole Cell Lysate, Lane 4: HMY2 Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- sCD40L antigen affinity purified polyclonal antibody (Catalog # PA1020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for sCD40L at approximately 29KD. The expected band size for sCD40L is at 29KD.
- Additional image