GTX22803

Submitted by: GeneTex (provider)
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Experimental details: Immunofluorescent analysis of Acetylcholinesterase in HeLa cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Acetylcholinesterase antibody [HR2] at a dilution of 1:200 over night at 4¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Immunofluorescent analysis of Acetylcholinesterase in U251 Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Acetylcholinesterase antibody [HR2] at a dilution of 1:200 over night at 4¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Immunofluorescent analysis of Acetylcholinesterase in Neuro-2a cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Acetylcholinesterase antibody [HR2] at a dilution of 1:200 over night at 4¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Immunohistochemistry was performed on normal deparaffinized human Cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed with or without Acetylcholinesterase antibody [HR2] at a dilution of 1:50 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Immunohistochemistry was performed on normal deparaffinized human Rectum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed with or without Acetylcholinesterase antibody [HR2] at a dilution of 1:20 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal deparaffinized human Brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed with or without Acetylcholinesterase antibody [HR2] at a dilution of at a dilution of 1:200 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.