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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F50830 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Acetylcholinesterase Antibody / ACHE
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human, Guinea Pig
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Storage
- Aliquot the Acetylcholinesterase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Acetylcholinesterase antibody western blot analysis in human 1) Jurkat, 2) Raji and 3) Y79 lysate. Predicted molecular weight ~68 kDa with a possible ~58 kDa isoform.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Acetylcholinesterase antibody with human NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments labeled with Alexa Fluor 555 Phalloidin (red). DAPI used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human brain tissue stained with Acetylcholinesterase antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Acetylcholinesterase antibody flow cytometric analysis of human NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
