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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF7628 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal PUM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse PUM1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PUM2 is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human and Mouse PUM1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, SW480 human colorectal adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PUM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7628) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PUM1 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
