AF7628

PUM1 antibody from Novus Biologicals

KIAA0099, PUMH1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Provider product page for AF7628

Antibody Data

Product number

AF7628

Provider

Novus Biologicals

Product name

Sheep Polyclonal PUM1 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse PUM1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PUM2 is observed.

Reactivity

Human, Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse PUM1 by Western Blot. Detection of Human and Mouse PUM1 by Western Blot.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse PUM1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, SW480 human colorectal adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PUM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7628) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PUM1 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.