26256-1-AP

antibody from Invitrogen Antibodies

Targeting: PUM1 KIAA0099, PUMH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

26256-1-AP

Provider

Invitrogen Antibodies

Product name

PUM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSVACVLKR KAVLWQDSFS PHLKHHPQEP ANPNMPVVLT SGTGSQAQPQ PAANQALAAG THSSPVPGSI GVAGRSQDDA MVDYFFQRQH GEQLGGGGSG GGGYNNSKHR WPTGDNIHAE HQVRSMDEL (1-128 aa encoded by BC013398 )

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 26256-1-AP ( PUM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 26256-1-AP (PUM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 26256-1-AP (PUM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PUM1 (IP:26256-1-AP, 4ug; Detection:26256-1-AP 1:300) with MCF-7 cells lysate 3200ug.