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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
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- Product number
- NB100-259 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-259, RRID:AB_2173593
- Product name
- Goat Polyclonal PUM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human γ-globin gene.
ABCA1 protein enhances Toll-like receptor 4 (TLR4)-stimulated interleukin-10 (IL-10) secretion through protein kinase A (PKA) activation.
Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function.
Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.
High-molecular-mass APOBEC3G complexes restrict Alu retrotransposition.
Zhu X, Wang Y, Pi W, Liu H, Wickrema A, Tuan D
PloS one 2012;7(10):e47175
PloS one 2012;7(10):e47175
ABCA1 protein enhances Toll-like receptor 4 (TLR4)-stimulated interleukin-10 (IL-10) secretion through protein kinase A (PKA) activation.
Ma L, Dong F, Zaid M, Kumar A, Zha X
The Journal of biological chemistry 2012 Nov 23;287(48):40502-12
The Journal of biological chemistry 2012 Nov 23;287(48):40502-12
Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function.
Vessey JP, Schoderboeck L, Gingl E, Luzi E, Riefler J, Di Leva F, Karra D, Thomas S, Kiebler MA, Macchi P
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3222-7
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3222-7
Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.
Morris AR, Mukherjee N, Keene JD
Molecular and cellular biology 2008 Jun;28(12):4093-103
Molecular and cellular biology 2008 Jun;28(12):4093-103
High-molecular-mass APOBEC3G complexes restrict Alu retrotransposition.
Chiu YL, Witkowska HE, Hall SC, Santiago M, Soros VB, Esnault C, Heidmann T, Greene WC
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 17;103(42):15588-93
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 17;103(42):15588-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUM1 Antibody [NB100-259] - Detection of Human and Mouse Pumillio 1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUM1 Antibody [NB100-259] - Analysis of human Pumilio 1, using NB100-259. Sample: RIPA extract (10 ug) from HeLa cells (CIP, calf intestinal phosphatase).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUM1 Antibody [NB100-259] - NIH-3T3 cell lysate (50 ug loaded), probed with Pumilio-1 antibody diluted at 0.1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody NB100-259 (lot NB100-259-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-259-2) and another rabbit anti-Pumillio 1 antibody. For blotting immunoprecipitated Pumillio 1, NB100-259 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 seconds.
