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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501034 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501034, RRID:AB_11126240
- Product name
- Anti-NIT1 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)
- Antibody type
- Monoclonal
- Description
- Anti-NIT1 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NIT1
- Isotype
- IgG
- Antibody clone number
- OTI4D12
- Vial size
- 100 µl
- Concentration
- 0.66 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NIT1 mouse monoclonal antibody (TA501034) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1(RC211519).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501034).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501034, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY NIT1(RC211519)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT1 mouse monoclonal(TA501034), and then analyzed by flow cytometry.
- Validation comment
- FC
