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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501084 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501084, RRID:AB_11125255
- Product name
- Anti-NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)
- Antibody type
- Monoclonal
- Description
- Anti-NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NIT1
- Isotype
- IgG
- Antibody clone number
- OTI3A11
- Vial size
- 100 µl
- Concentration
- 0.87 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-NIT1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NIT1 mouse monoclonal antibody (TA501084) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1(RC211519).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501084).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501084, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501084, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY NIT1(RC211519)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT1 mouse monoclonal(TA501084), and then analyzed by flow cytometry.
- Validation comment
- FC
