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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00004817-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004817-M01, RRID:AB_2151312
- Product name
- Mouse Monoclonal Nitrilase 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Reacts with nitrilase 1.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of monoclonal antibody to NIT1 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
