14685-1-AP

antibody from Proteintech Group

Targeting: PAK4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

14685-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14685-1-AP, RRID:AB_2158610

Product name

PAK4 antibody

Antibody type

Polyclonal

Description

KD/KO validated PAK4 antibody (Cat. #14685-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation.

Yang R, Qu X, Zhi S, Wang J, Fu J, Tan C, Chen H, Wang X
Molecular neurobiology 2024 Sep;61(9):7195-7210

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Dual-inhibition of NAMPT and PAK4 induces anti-tumor effects in 3D-spheroids model of platinum-resistant ovarian cancer.

Kudo K, Greer YE, Yoshida T, Harrington BS, Korrapati S, Shibuya Y, Henegar L, Kopp JB, Fujii T, Lipkowitz S, Annunziata CM
Cancer gene therapy 2024 May;31(5):721-735

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Expression Patterns of PAK4 and PHF8 Are Associated with the Survival of Gallbladder Carcinoma Patients.

Ahn AR, Karamikheirabad M, Park MS, Zhang J, Kim HS, Jeong JS, Kim KM, Park HS, Jang KY
Diagnostics (Basel, Switzerland) 2023 Mar 17;13(6)

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Pan-cancer analysis identifies the immunological and prognostic role of PAK4.

Tang L, Gao Y, Li T
Life sciences 2023 Jan 1;312:121263

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Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation.

Wang DN, Ni JJ, Li JH, Gao YQ, Ni FJ, Zhang ZZ, Fang JY, Lu J, Yao YF
PLoS pathogens 2023 Feb;19(2):e1011189

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RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling.

Zhu H, Wen Z, Zhang A, Liu D, Wang H, Cheng Y, Yang X, Xiao Y, Li J, Sun D, Wu B, Gao J
Communications biology 2023 Feb 23;6(1):214

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Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p.

Zhong J, Xu A, Xu P, Su M, Wang P, Liu Z, Li B, Liu C, Jiang N
Cell death discovery 2023 Aug 2;9(1):283

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The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.

Schiavi-Ehrenhaus LJ, Romarowski A, Jabloñski M, Krapf D, Luque GM, Buffone MG
The Journal of biological chemistry 2022 Jun;298(6):101988

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Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.

Baskaran Y, Tay FP, Ng EYW, Swa CLF, Wee S, Gunaratne J, Manser E
Nature communications 2021 Sep 7;12(1):5315

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Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma.

Dasgupta A, Sierra L, Tsang SV, Kurenbekova L, Patel T, Rajapakse K, Shuck RL, Rainusso N, Landesman Y, Unger T, Coarfa C, Yustein JT
Cancer research 2021 Jan 1;81(1):199-212

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PAK4 inhibition improves PD-1 blockade immunotherapy.

Abril-Rodriguez G, Torrejon DY, Liu W, Zaretsky JM, Nowicki TS, Tsoi J, Puig-Saus C, Baselga-Carretero I, Medina E, Quist MJ, Garcia AJ, Senapedis W, Baloglu E, Kalbasi A, Cheung-Lau G, Berent-Maoz B, Comin-Anduix B, Hu-Lieskovan S, Wang CY, Grasso CS, Ribas A
Nature cancer 2020;1(1):46-58

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PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.

Zhang S, Yin Y, Li C, Zhao Y, Wang Q, Zhang X
Aging 2020 Jul 12;12(13):12740-12749

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ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Meng L, Liu S, Liu F, Sang M, Ju Y, Fan X, Gu L, Li Z, Geng C, Sang M
Molecular therapy. Nucleic acids 2020 Aug 19;22:124-137

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Nonconserved miR-608 suppresses prostate cancer progression through RAC2/PAK4/LIMK1 and BCL2L1/caspase-3 pathways by targeting the 3'-UTRs of RAC2/BCL2L1 and the coding region of PAK4.

Zhang X, Fang J, Chen S, Wang W, Meng S, Liu B
Cancer medicine 2019 Sep;8(12):5716-5734

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Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion.

Rabieifar P, Zhuang T, Costa TDF, Zhao M, Strömblad S
Scientific reports 2019 Oct 8;9(1):14436

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Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits.

Katrancha SM, Shaw JE, Zhao AY, Myers SA, Cocco AR, Jeng AT, Zhu M, Pittenger C, Greer CA, Carr SA, Xiao X, Koleske AJ
Cell reports 2019 Mar 5;26(10):2805-2817.e9

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CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.

Miura K, Nojiri T, Akitake Y, Ando K, Fukuhara S, Zenitani M, Kimura T, Hino J, Miyazato M, Hosoda H, Kangawa K
The Biochemical journal 2017 May 19;474(11):1897-1918

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MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Sheng N, Tan G, You W, Chen H, Gong J, Chen D, Zhang H, Wang Z
Cancer medicine 2017 Jun;6(6):1331-1340

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The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity.

Selamat W, Tay PL, Baskaran Y, Manser E
PloS one 2015;10(6):e0129634

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP(PAK4 antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PAK4 antibody 14685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 14685-1-AP(PAK4 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 14685-1-AP(PAK4 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue