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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- GTX32289 - Provider product page
- Provider
- GeneTex
- Product name
- PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references P21-activated kinase 4 in pancreatic acinar cells is activated by numerous gastrointestinal hormones/neurotransmitters and growth factors by novel signaling, and its activation stimulates secretory/growth cascades.
Ramos-Alvarez I, Jensen RT
American journal of physiology. Gastrointestinal and liver physiology 2018 Aug 1;315(2):G302-G317
American journal of physiology. Gastrointestinal and liver physiology 2018 Aug 1;315(2):G302-G317
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of PAK4 (pS474) expression in HEK293T EGF-treated (A); Raw264.7 EGF-treated (B); PC12 EGF-treated (C) whole cell lysates.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
