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Learn14685-1-AP
antibody from Invitrogen Antibodies
Targeting: PAK4
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14685-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MFGKRKKRV EISAPSNFEH RVHTGFDQHE QKFTGLPRQW QSLIEESARR PKPLVDPACI TSIQPGAPKT IVRGSKGAKD GALTLLLDEF ENMSVTRSNS LRRDSPPPPA RARQENGMPE EPATTARGGP GKAGSRGRFA GHSEAGGGSG DRRRAGPEKR PKSSREGSGG PQESSRDKRP LSGPDVGTPQ PAGLASGAKL AAGRPFNTYP RADTDHPSRG AQGEPHDVAP NGPSAGGLAI PQSSSSSSRP PTRARGAPSP GVLGPHASEP QLAPPACTPA APAVPGPPGP RSPQREPQRV SHEQFRAALQ LVVDPGDPRS YLDNFIKIGE GSTGIVCIAT VRSSGKLVAV K (1-350 aa encoded by BC025282)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.51 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of PAK4 antibody (14685-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK4 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat skin tissue were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PAK4 antibody 14685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14685-1-AP (PAK4 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14685-1-AP (PAK4 antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug PAK4 antibody (14685-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PAK4 (IP:14685-1-AP, 4ug; Detection:14685-1-AP 1:800) with mouse liver tissue lysate 4000ug.
