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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-16257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-S100 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- PA5-16265 targets Chorionic Gonadotropin Human in IHC (P) applications and shows reactivity with Human samples. The PA5-16265 immunogen is purified Beta-subunit of HCG.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C
Submitted references Expression of cellular components in granulomatous inflammatory response in Piaractus mesopotamicus model.
Papillary-cystic pattern is characteristic in mammary analogue secretory carcinomas but is rarely observed in acinic cell carcinomas of the salivary gland.
Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice.
MT1-MMP modulates melanoma cell dissemination and metastasis through activation of MMP2 and RAC1.
Cortical hypoplasia and ventriculomegaly of p73-deficient mice: Developmental and adult analysis.
A novel electrospun nerve conduit enhanced by carbon nanotubes for peripheral nerve regeneration.
Low-copy piggyBac transposon mutagenesis in mice identifies genes driving melanoma.
Expression of insulin-like growth factor binding proteins during mouse cochlear development.
A microscale neuron and Schwann cell coculture model for increasing detection sensitivity of botulinum neurotoxin type A.
Cutaneous innervation of the human face as assessed by skin biopsy.
Role of the neuroglia of human ocular transparent structures in the visual perception concepts.
Veil nerve-sparing technique and postoperative urinary continence in open antegrade radical prostatectomy.
SPARC-like 1 (SC1) is a diversely expressed and developmentally regulated matricellular protein that does not compensate for the absence of SPARC in the CNS.
A case of xanthoma in a Saanen goat.
Manrique WG, Claudiano Gda S, de Castro MP, Petrillo TR, Pereira Figueiredo MA, Belo MA, Berdeal MI, de Moraes JE, de Moraes FR
PloS one 2015;10(3):e0121625
PloS one 2015;10(3):e0121625
Papillary-cystic pattern is characteristic in mammary analogue secretory carcinomas but is rarely observed in acinic cell carcinomas of the salivary gland.
Hsieh MS, Chou YH, Yeh SJ, Chang YL
Virchows Archiv : an international journal of pathology 2015 Aug;467(2):145-53
Virchows Archiv : an international journal of pathology 2015 Aug;467(2):145-53
Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice.
Gombash SE, Cowley CJ, Fitzgerald JA, Hall JC, Mueller C, Christofi FL, Foust KD
Frontiers in molecular neuroscience 2014;7:81
Frontiers in molecular neuroscience 2014;7:81
MT1-MMP modulates melanoma cell dissemination and metastasis through activation of MMP2 and RAC1.
Shaverdashvili K, Wong P, Ma J, Zhang K, Osman I, Bedogni B
Pigment cell & melanoma research 2014 Mar;27(2):287-96
Pigment cell & melanoma research 2014 Mar;27(2):287-96
Cortical hypoplasia and ventriculomegaly of p73-deficient mice: Developmental and adult analysis.
Medina-Bolívar C, González-Arnay E, Talos F, González-Gómez M, Moll UM, Meyer G
The Journal of comparative neurology 2014 Aug 1;522(11):2663-79
The Journal of comparative neurology 2014 Aug 1;522(11):2663-79
A novel electrospun nerve conduit enhanced by carbon nanotubes for peripheral nerve regeneration.
Yu W, Jiang X, Cai M, Zhao W, Ye D, Zhou Y, Zhu C, Zhang X, Lu X, Zhang Z
Nanotechnology 2014 Apr 25;25(16):165102
Nanotechnology 2014 Apr 25;25(16):165102
Low-copy piggyBac transposon mutagenesis in mice identifies genes driving melanoma.
Ni TK, Landrette SF, Bjornson RD, Bosenberg MW, Xu T
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 17;110(38):E3640-9
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 17;110(38):E3640-9
Expression of insulin-like growth factor binding proteins during mouse cochlear development.
Okano T, Kelley MW
Developmental dynamics : an official publication of the American Association of Anatomists 2013 Oct;242(10):1210-21
Developmental dynamics : an official publication of the American Association of Anatomists 2013 Oct;242(10):1210-21
A microscale neuron and Schwann cell coculture model for increasing detection sensitivity of botulinum neurotoxin type A.
Hong WS, Young EW, Tepp WH, Johnson EA, Beebe DJ
Toxicological sciences : an official journal of the Society of Toxicology 2013 Jul;134(1):64-72
Toxicological sciences : an official journal of the Society of Toxicology 2013 Jul;134(1):64-72
Cutaneous innervation of the human face as assessed by skin biopsy.
Nolano M, Provitera V, Caporaso G, Stancanelli A, Leandri M, Biasiotta A, Cruccu G, Santoro L, Truini A
Journal of anatomy 2013 Feb;222(2):161-9
Journal of anatomy 2013 Feb;222(2):161-9
Role of the neuroglia of human ocular transparent structures in the visual perception concepts.
Reva GV, Kovaleva IV, Reva IV, Yamamoto T, Novikov AS, Lomakin AV, Kulikova ES
Bulletin of experimental biology and medicine 2013 Feb;154(4):515-20
Bulletin of experimental biology and medicine 2013 Feb;154(4):515-20
Veil nerve-sparing technique and postoperative urinary continence in open antegrade radical prostatectomy.
Shigemura K, Yamanaka N, Yamashita M
Urologia internationalis 2012;89(3):283-9
Urologia internationalis 2012;89(3):283-9
SPARC-like 1 (SC1) is a diversely expressed and developmentally regulated matricellular protein that does not compensate for the absence of SPARC in the CNS.
Lloyd-Burton S, Roskams AJ
The Journal of comparative neurology 2012 Aug 15;520(12):2575-90
The Journal of comparative neurology 2012 Aug 15;520(12):2575-90
A case of xanthoma in a Saanen goat.
Ozmen O, Haligur M
Veterinary dermatology 2012 Apr;23(2):150-2, e32
Veterinary dermatology 2012 Apr;23(2):150-2, e32
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of S100 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with S100 Rabbit Polyclonal Antibody (Product # PA5-16257) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human melanoma stained with S-100 antibody using peroxidase-conjugate and AEC chromogen. Note intense cytoplasmic staining of tumor cells.
