- PA1-934A - Invitrogen Antibodies CASR antibody

Submitted references Calcium-Sensing Receptor Contributes to Hyperoxia Effects on Human Fetal Airway Smooth Muscle.

Roesler AM, Ravix J, Bartman CM, Patel BS, Schiliro M, Roos B, Nesbitt L, Pabelick CM, Martin RJ, MacFarlane PM, Prakash YS
Frontiers in physiology 2021;12:585895

Calcium sensing receptor in developing human airway smooth muscle.

Roesler AM, Wicher SA, Ravix J, Britt RD Jr, Manlove L, Teske JJ, Cummings K, Thompson MA, Farver C, MacFarlane P, Pabelick CM, Prakash YS
Journal of cellular physiology 2019 Aug;234(8):14187-14197

Evidence for a regulated Ca(2+) entry in proximal tubular cells and its implication in calcium stone formation.

Ibeh CL, Yiu AJ, Kanaras YL, Paal E, Birnbaumer L, Jose PA, Bandyopadhyay BC
Journal of cell science 2019 Apr 30;132(9)

Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study.

Baio G, Rescinito G, Rosa F, Pace D, Boccardo S, Basso L, Salvi S, Calabrese M, Truini M, Neumaier CE
Molecular imaging and biology 2015 Aug;17(4):548-56

Decreased parathyroid Klotho expression is associated with persistent hyperparathyroidism after kidney transplantation.

Hong YA, Choi DE, Lim SW, Yang CW, Chang YK
Transplantation proceedings 2013 Oct;45(8):2957-62

A novel germline inactivating mutation in the CASR gene in an Italian kindred affected by familial hypocalciuric hypercalcemia.

Falchetti A, Gozzini A, Terranegra A, Soldati L, Vezzoli G, Leoncini G, Giusti F, Franceschelli F, Masi L, Tanini A, Cavalli L, Brandi ML
European journal of endocrinology 2012 May;166(5):933-40

In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.

Baio G, Fabbi M, Emionite L, Cilli M, Salvi S, Ghedin P, Prato S, Carbotti G, Tagliafico A, Truini M, Neumaier CE
European radiology 2012 Mar;22(3):551-8

Stimulation of the calcium-sensing receptor stabilizes the podocyte cytoskeleton, improves cell survival, and reduces toxin-induced glomerulosclerosis.

Oh J, Beckmann J, Bloch J, Hettgen V, Mueller J, Li L, Hoemme M, Gross ML, Penzel R, Mundel P, Schaefer F, Schmitt CP
Kidney international 2011 Sep;80(5):483-92

Calcium-dependent diuretic system in preascitic liver cirrhosis.

Sansoè G, Aragno M, Tomasinelli CE, di Bonzo LV, Wong F, Parola M
Journal of hepatology 2010 Nov;53(5):856-62

Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.

Ishikawa M, Kimura K, Tachibana T, Hashimoto H, Shimojo M, Ueshiba H, Tsuboi K, Shibuya K, Yoshino G
Human cell 2010 May;23(2):58-64

Regulation of mouse lung development by the extracellular calcium-sensing receptor, CaR.

Finney BA, del Moral PM, Wilkinson WJ, Cayzac S, Cole M, Warburton D, Kemp PJ, Riccardi D
The Journal of physiology 2008 Dec 15;586(24):6007-19

Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands.

Yang AH, Hsu CW, Chen JY, Tseng LM, Won GS, Lee CH
Virchows Archiv : an international journal of pathology 2006 Jul;449(1):62-8

The calcium sensing receptor and its alternatively spliced form in keratinocyte differentiation.

Oda Y, Tu CL, Pillai S, Bikle DD
The Journal of biological chemistry 1998 Sep 4;273(36):23344-52

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Calcium Sensing Receptor was performed by loading 25 µg of U251 (lane 1) and rat brain (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~120 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of PC12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Hyperoxia effect on expression of the extracellular Ca 2+ ([Ca 2+ ] o ) sensing receptor (CaSR) in developing human fetal (

PA1-934A

Antibody data