AP2890c

antibody from Abgent

Targeting: CCT3 Cctg, TRIC5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2890c

Provider

Abgent

Proper citation

Abgent Cat#AP2890c, RRID:AB_1967379

Product name

CCT3 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of CCT3 Antibody (Center) (Cat. #AP2890c) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) (Cat. #AP2890c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) .

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50