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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21968 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibrinogen gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, human plasma, HepG2 sup., mouse plasma, rat plasma. Predicted reactivity: Mouse (85%), Rat (85%), Pig (84%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.77 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fibrinogen gamma using 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Fibrinogen gamma polyclonal antibody (Product # PA5-21968) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fibrinogen gamma in Human plasma and HepG2 conditioned medium. Samples were separated by 10% SDS-PAGE and the membrane was probed with Fibrinogen gamma Polyclonal antibody (Product # PA5-21968) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fibrinogen gamma was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968). Human plasma and HepG2 conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968). Mouse plasma and rat plasma (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968) diluted at 1:20,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Fibrinogen gamma was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma Polyclonal Antibody (Product # PA5 21968) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fibrinogen gamma Polyclonal Antibody detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma stained by Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fibrinogen gamma Polyclonal Antibody detects FGG protein at cytosol on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968) dilution: 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Fibrinogen gamma was performed in HepG2 whole cell extracts using 5 µg of Fibrinogen gamma Polyclonal Antibody (Product # PA5-21968). Samples were transferred to a membrane and probed with Fibrinogen gamma Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
