Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA325083 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal GC Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal GC Antibody (Center)
- Reactivity
- Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GBA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.345 mg/ml
Submitted references Validation of anti-glucocerebrosidase antibodies for western blot analysis on protein lysates of murine and human cells.
Qi W, Davidson BA, Nguyen M, Lindstrom T, Grey RJ, Burnett R, Aflaki E, Sidransky E, Westbroek W
The Biochemical journal 2019 Jan 25;476(2):261-274
The Biochemical journal 2019 Jan 25;476(2):261-274
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of GC Antibody (Center) (Cat. #TA325083) in mouse lung tissue lysates (35ug/lane). GC (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human Skin reacted with GC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GC Antibody (Center) (Cat. #TA325083) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC