PA5-111798

antibody from Invitrogen Antibodies

Targeting: KCNJ10 Kir1.2, Kir4.1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-111798

Provider

Invitrogen Antibodies

Product name

Kir4.1 (KCNJ10) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Guinea Pig

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Kir4.1 (KCNJ10) was performed in rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6). Lane 1-3: Kir4.1 (KCNJ10) Antibody (Product # PA5-111798) at a dilution of 1:800. Lane 4-6: Kir4.1 (KCNJ10) Antibody preincubated with the negative control antigen.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Kir4.1 (KCNJ10) was performed in rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6). Lane 1-3: Kir4.1 (KCNJ10) Antibody (Product # PA5-111798) at a dilution of 1:800. Lane 4-6: Kir4.1 (KCNJ10) Antibody preincubated with the negative control antigen.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Kir4.1 (KCNJ10) in fixed and permeabilized human U-87 MG glioblastoma cell line using Kir4.1 (KCNJ10) Antibody (Product # PA5-111798), (1:100) followed by goat anti-guinea pig-AlexaFluor-488 secondary antibody (green). Cell nuclei were visualized using Hoechst 33342 (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Kir4.1 (KCNJ10) in frozen rat hippocampal dentate gyrus tissue sections using Kir4.1 (KCNJ10) Antibody (Product # PA5-111798) at a dilution of 1:300. A) Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer (Mol). B) The same section was stained with mouse anti-glial fibrillary acidic protein (GFAP), (red) showing astrocyte profiles. C) Merge of Kir4.1 and GFAP images reveals colocalization on some branches of astrocytic fibers. In addition, Kir4.1 is expressed in the fine ramification of astrocytic fibers, thus creating a “halo-like8221 cloud around the GFAP astrocytic outline. DAPI (blue) counterstain reveals the outline of the dentate granule layer.