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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 16511-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUPT5H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: PLHDGSRTP AQSGAWDPNN PNTPSRAEEE YEYAFDDEPT PSPQAYGGTP NPQTPGYPDP SSPQVNPQYN PQTPGTPAMY NTDQFSPYAA PSPQGSYQPS PSPQSYHQVA PSPAGYQNTH SPASYHPTPS PMAYQASPSP SPVGYSPMTP GAPSPGGYNP HTPGSGIEQN SSDWVTTDIQ VKVRDTYLDT QVVGQTGVIR SVTGGMCSVY LKDSEKVVSI SSEHLEPITP TKNNKVKVIL GEDREATGVL LSIDGEDGIV RMDLDEQLKI LNLRFLGKLL EA (807-1087 aa encoded by BC024203)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16511-1-AP ( SUPT5H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16511-1-AP (SUPT5H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 16511-1-AP (SUPT5H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SUPT5H (IP:16511-1-AP, 4ug; Detection:16511-1-AP 1:500) with HeLa cells lysate 3200ug.
