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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- 65689ACTMOTIF - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Supt5H/SPT5 Monoclonal Antibody (6F1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 6F1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supt5H/SPT5 antibody (mAb) tested by Western blot 20 µg of K562 nuclear extract was run on SDS-PAGE and probed with antibody at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supt5H/SPT5 antibody (mAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 µg of K562 cell chromatin and 4 µg of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify Supt5H binding sites on chromosome 19.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supt5H/SPT5 antibody (mAb) tested by Immunoprecipitation. 5 µg of Supt5H antibody (Lane 1) or an unrelated IgG (Lane 2) was used to immunoprecipitate Supt5H from 300 µg of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a the same Supt5H antibody at 2 µg/mL (crossreaction between heavy and light chains of the antibody is observed and is common).
