- 39-6100 - Invitrogen Antibodies TACR1 antibody

Submitted references Respiratory disturbances in a mouse model of Parkinson's disease.

Oliveira LM, Oliveira MA, Moriya HT, Moreira TS, Takakura AC
Experimental physiology 2019 May;104(5):729-739

Human microglia and astrocytes constitutively express the neurokinin-1 receptor and functionally respond to substance P.

Burmeister AR, Johnson MB, Chauhan VS, Moerdyk-Schauwecker MJ, Young AD, Cooley ID, Martinez AN, Ramesh G, Philipp MT, Marriott I
Journal of neuroinflammation 2017 Dec 13;14(1):245

Cyclophosphamide causes activation of protein kinase A (PKA) in the brainstem of vomiting least shrews (Cryptotis parva).

Alkam T, Chebolu S, Darmani NA
European journal of pharmacology 2014 Jan 5;722:156-64

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Neuro-2a (Lane 1) and A-431 (Lane 2). The blots were probed with Anti-Neurokinin 1 Receptor/NK1R Mouse Monoclonal Antibody (Product # 39-6100, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 50 kDa band corresponding to Neurokinin 1 Receptor/NK1R was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), Neuro-2A (Lane 2), A-431 (Lane 3), tissue extract of Mouse Brain (Lane 4), whole cell extracts of Caco-2 (Lane 5), MCF7 (Lane 6), NTERA-2 cl,D1 (Lane 7), SK-N-AS (Lane 8) and Hep G2 (Lane 9). The blot was probed with Anti- TACR1 Monoclonal Antibody (ZN003) (Product # 39-6100, 1µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 50 kDa band corresponding to TACR1 was observed across the cell lines and tissue tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SF9 cell lysates using Zymed Ms anti-NK1 Receptor (Product # 39-6100).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Neurokinin 1 Receptor / NK1R Receptor was performed using 90% confluent log phase Neuro 2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurokinin 1 Receptor / NK1R (ZN003) Mouse Monoclonal Antibody (Product # 39-6100) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Neurokinin 1 Receptor / NK1R was done on Neuro-2a cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Neurokinin 1 Receptor / NK1R Mouse Monoclonal Antibody (Product # 39-6100, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

39-6100

Antibody data