PA1-32229

antibody from Invitrogen Antibodies

Targeting: TACR1 NK1R, NKIR, SPR, TAC1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-32229

Provider

Invitrogen Antibodies

Product name

TACR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Canine, Guinea Pig

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Activation of neuroimmune pathways increases therapeutic effects of radiotherapy on poorly differentiated breast carcinoma.

Erin N, Korcum AF, Tanrıöver G, Kale Ş, Demir N, Köksoy S
Brain, behavior, and immunity 2015 Aug;48:174-85

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Identification of clinical target areas in the brainstem of prion-infected mice.

Mirabile I, Jat PS, Brandner S, Collinge J
Neuropathology and applied neurobiology 2015 Aug;41(5):613-30

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TACR1 Polyclonal Antibody (Product # PA1-32229) and 36 kDa bands corresponding to TACR1 were observed across cell lines tested except in MIA-PaCa-2 and SK-BR-3, which are reported to be low expressing for TACR1. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), PANC-1 (Lane 2), MIA-PaCa-2 (Lane 3), SK-BR-3 (Lane 4), MCF7 (Lane 5), MDA-MB-231 (Lane 6), SK-OV-3 (Lane 7) and NIH: OVCAR-3 (Lane 8) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TACR1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TACR1 Polyclonal Antibody (Product PA1-32229) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membrane localization of TACR1. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TACR1 in mouse brain (selected cells, axon and dendrites) tissue using TACR1 Polyclonal Antibody (Product # PA1-32229).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TACR1 in rat spinal cord tissue using TACR1 Polyclonal Antibody (Product # PA1-32229).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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