NB300-119

antibody from Novus Biologicals

Targeting: TACR1 NK1R, NKIR, SPR, TAC1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NB300-119

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-119, RRID:AB_10002802

Product name

Rabbit Polyclonal NK1R Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Neurokinin-1 Receptor Signaling Is Required for Efficient Ca²⁺ Flux in T-Cell-Receptor-Activated T Cells.

Morelli AE, Sumpter TL, Rojas-Canales DM, Bandyopadhyay M, Chen Z, Tkacheva O, Shufesky WJ, Wallace CT, Watkins SC, Berger A, Paige CJ, Falo LD Jr, Larregina AT
Cell reports 2020 Mar 10;30(10):3448-3465.e8

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miR-206 Promotes Cancer Progression by Targeting Full-Length Neurokinin-1 Receptor in Breast Cancer.

Zhou Y, Wang M, Tong Y, Liu X, Zhang L, Dong D, Shao J, Zhou Y
Technology in cancer research & treatment 2019 Jan 1;18:1533033819875168

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Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice.

Li Q, Wu X, Yang Y, Zhang Y, He F, Xu X, Zhang Z, Tao L, Luo C
The international journal of biochemistry & cell biology 2019 Feb;107:154-165

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Varicella Zoster Virus Induces Nuclear Translocation of the Neurokinin-1 Receptor, Promoting Lamellipodia Formation and Viral Spread in Spinal Astrocytes.

Bubak AN, Como CN, Blackmon AM, Frietze S, Mescher T, Jones D, Cohrs RJ, Paucek P, Baird NL, Nagel MA
The Journal of infectious diseases 2018 Sep 8;218(8):1324-1335

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Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits.

Wang D, Tawfik VL, Corder G, Low SA, François A, Basbaum AI, Scherrer G
Neuron 2018 Apr 4;98(1):90-108.e5

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Up-regulated expression of substance P in CD8⁺ T cells and NK1R on monocytes of atopic dermatitis.

Zhang Z, Zheng W, Xie H, Chai R, Wang J, Zhang H, He S
Journal of translational medicine 2017 May 1;15(1):93

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Targeting tachykinin receptors in neuroblastoma.

Henssen AG, Odersky A, Szymansky A, Seiler M, Althoff K, Beckers A, Speleman F, Schäfers S, De Preter K, Astrahanseff K, Struck J, Schramm A, Eggert A, Bergmann A, Schulte JH
Oncotarget 2017 Jan 3;8(1):430-443

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Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells.

Zhou Y, Zuo D, Wang M, Zhang Y, Yu M, Yang J, Yao Z
Genes to cells : devoted to molecular & cellular mechanisms 2014 Sep;19(9):676-91

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Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria.

Fujisawa D, Kashiwakura J, Kita H, Kikukawa Y, Fujitani Y, Sasaki-Sakamoto T, Kuroda K, Nunomura S, Hayama K, Terui T, Ra C, Okayama Y
The Journal of allergy and clinical immunology 2014 Sep;134(3):622-633.e9

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Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor.

Schwartz L, Spitsin SV, Meshki J, Tuluc F, Douglas SD, Wolfe JH
Journal of neurovirology 2013 Jun;19(3):219-27

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Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer.

Zhou Y, Zhao L, Xiong T, Chen X, Zhang Y, Yu M, Yang J, Yao Z
Breast cancer research and treatment 2013 Jul;140(1):49-61

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Kinetics of neuronal contribution during the development of a contact allergic reaction.

Altawil R, Lyström J, El-Nour H
Archives of dermatological research 2012 May;304(4):273-81

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Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation.

Humes DJ, Simpson J, Smith J, Sutton P, Zaitoun A, Bush D, Bennett A, Scholefield JH, Spiller RC
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2012 Apr;24(4):318-e163

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GABA(A) Receptor β3 Subunit Expression Regulates Tonic Current in Developing Striatopallidal Medium Spiny Neurons.

Janssen MJ, Yasuda RP, Vicini S
Frontiers in cellular neuroscience 2011;5:15

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Cardiac damage after lesions of the nucleus tractus solitarii.

Nayate A, Moore SA, Weiss R, Taktakishvili OM, Lin LH, Talman WT
American journal of physiology. Regulatory, integrative and comparative physiology 2009 Feb;296(2):R272-9

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Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon.

Andersson G, Danielson P, Alfredson H, Forsgren S
Regulatory peptides 2008 Oct 9;150(1-3):81-7

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The fate of the large striatal interneurons expressing calretinin in Huntington's disease.

Massouh M, Wallman MJ, Pourcher E, Parent A
Neuroscience research 2008 Dec;62(4):216-24

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cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I.

Schmidtko A, Gao W, König P, Heine S, Motterlini R, Ruth P, Schlossmann J, Koesling D, Niederberger E, Tegeder I, Friebe A, Geisslinger G
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Aug 20;28(34):8568-76

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Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the NK-1 receptor.

Grimsholm O, Rantapää-Dahlqvist S, Dalén T, Forsgren S
Neuropeptides 2008 Apr;42(2):133-45

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Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis.

Andersson G, Danielson P, Alfredson H, Forsgren S
Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA 2007 Oct;15(10):1272-9

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New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout.

Ferguson C, Hardy SL, Werner DF, Hileman SM, Delorey TM, Homanics GE
BMC neuroscience 2007 Oct 10;8:85

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Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra.

Lévesque M, Wallman MJ, Parent R, Sík A, Parent A
Neuroscience research 2007 Mar;57(3):362-71

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Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection.

Hazlett LD, McClellan SA, Barrett RP, Liu J, Zhang Y, Lighvani S
Investigative ophthalmology & visual science 2007 Feb;48(2):797-807

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Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea.

Fong AY, Potts JT
The Journal of physiology 2006 Sep 15;575(Pt 3):869-85

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An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice.

Borghese CM, Werner DF, Topf N, Baron NV, Henderson LA, Boehm SL 2nd, Blednov YA, Saad A, Dai S, Pearce RA, Harris RA, Homanics GE, Harrison NL
The Journal of pharmacology and experimental therapeutics 2006 Oct;319(1):208-18

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Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus.

Lévesque M, Parent R, Parent A
The European journal of neuroscience 2006 May;23(10):2760-72

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Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation.

Lai JP, Ho WZ, Kilpatrick LE, Wang X, Tuluc F, Korchak HM, Douglas SD
Proceedings of the National Academy of Sciences of the United States of America 2006 May 16;103(20):7771-6

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Substance P and the neurokinin-1 receptor in relation to eosinophilia in ulcerative colitis.

Jönsson M, Norrgård O, Forsgren S
Peptides 2005 May;26(5):799-814

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Vascular NK-1 receptor occurrence in normal and chronic painful Achilles and patellar tendons: studies on chemically unfixed as well as fixed specimens.

Forsgren S, Danielson P, Alfredson H
Regulatory peptides 2005 Mar 30;126(3):173-81

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Expression of the NK-1 receptor on islet cells and invading immune cells in the non-obese diabetic mouse.

Persson-Sjögren S, Lejon K, Holmberg D, Forsgren S
Journal of autoimmunity 2005 Jun;24(4):269-79

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Depletion of ventromedullary NK-1 receptor-immunoreactive neurons in multiple system atrophy.

Benarroch EE, Schmeichel AM, Low PA, Parisi JE
Brain : a journal of neurology 2003 Oct;126(Pt 10):2183-90

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Increased tachykinin NK(1) receptor immunoreactivity in the prefrontal cortex in schizophrenia.

Tooney PA, Crawter VC, Chahl LA
Biological psychiatry 2001 Mar 15;49(6):523-7

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Increased tachykinin NK(1) receptor immunoreactivity in the prefrontal cortex in schizophrenia.

Tooney PA, Crawter VC, Chahl LA
Biological psychiatry 2001 Mar 15;49(6):523-7

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NK-1 receptor gene expression is related to pain in chronic pancreatitis.

Shrikhande SV, Friess H, di Mola FF, Tempia-Caliera A, Conejo Garcia JR, Zhu Z, Zimmermann A, Büchler MW
Pain 2001 Apr;91(3):209-17

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NK-1 receptor gene expression is related to pain in chronic pancreatitis.

Shrikhande SV, Friess H, di Mola FF, Tempia-Caliera A, Conejo Garcia JR, Zhu Z, Zimmermann A, Büchler MW
Pain 2001 Apr;91(3):209-17

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Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex.

Tooney PA, Au GG, Chahl LA
Neuroscience letters 2000 Apr 14;283(3):185-8

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Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex.

Tooney PA, Au GG, Chahl LA
Neuroscience letters 2000 Apr 14;283(3):185-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.