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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-35983 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOX3 using 30 µg MCF-7 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SOX3 polyclonal antibody (Product # PA5-35983) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOX3 Polyclonal Antibody (Product # PA5-35983). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX3 Polyclonal Antibody (Product # PA5-35983) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SOX3 showing staining in the nucleus of MCF-7 cells. MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SOX3 polyclonal antibody (Product # PA5-35983) diluted at 1:1000. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SOX3 was performed in U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SOX3 Polyclonal Antibody (Product # PA5-35983) diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SOX3 was performed in paraffin-embedded mouse brain tissue using SOX3 Polyclonal Antibody (Product # PA5-35983) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SOX3 was performed in paraffin-embedded mouse brain tissue using SOX3 Polyclonal Antibody (Product # PA5-35983) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SOX3 was performed in paraffin-embedded rat brain tissue using Green: Histone H3 Polyclonal Antibody (Product # PA5-31954) at a dilution of 1:200. The signal was developed using a goat anti-rabbit IgG antibody.
