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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF2569 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SOX3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human SOX3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX7, rhSOX10, and rhSOX17 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P41225- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identification of dynamic undifferentiated cell states within the male germline.
Identification of SOX3 as an XX male sex reversal gene in mice and humans.
La HM, Mäkelä JA, Chan AL, Rossello FJ, Nefzger CM, Legrand JMD, De Seram M, Polo JM, Hobbs RM
Nature communications 2018 Jul 19;9(1):2819
Nature communications 2018 Jul 19;9(1):2819
Identification of SOX3 as an XX male sex reversal gene in mice and humans.
Sutton E, Hughes J, White S, Sekido R, Tan J, Arboleda V, Rogers N, Knower K, Rowley L, Eyre H, Rizzoti K, McAninch D, Goncalves J, Slee J, Turbitt E, Bruno D, Bengtsson H, Harley V, Vilain E, Sinclair A, Lovell-Badge R, Thomas P
The Journal of clinical investigation 2011 Jan;121(1):328-41
The Journal of clinical investigation 2011 Jan;121(1):328-41
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- SOX3 in NTera-2 Human Cell Line. SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
