MA5-34906

antibody from Invitrogen Antibodies

Targeting: PRDX6 1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34906

Provider

Invitrogen Antibodies

Product name

PRDX6 Monoclonal Antibody (7G1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: PC-3M, K562, A431, SiHa, human liver tissue, human colon cancer tissue, human kidney tissue, human placenta tissue.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7G1

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRDX6 in PC-3M (1) and K562 (2). Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Peroxiredoxin-6 was performed by loading 25 µg of WT (lane 1) and PRDX6 CRISPR KO (lane 2) HAP1 cell lysates in RIPA buffer onto a 8-16% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. PRDX6 was detected at approximately 25 kDa (designated by the black arrow) using a PRDX6 monoclonal antibody (Product # MA5-34906) at a dilution of 1:500 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 62-6520) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PRDX6 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRDX6 monoclonal antibody (Product # MA5-34906), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PRDX6 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRDX6 monoclonal antibody (Product # MA5-34906), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PRDX6 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRDX6 monoclonal antibody (Product # MA5-34906), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRDX6 in paraffin-embedded human placenta tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRDX6 in paraffin-embedded human placenta tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRDX6 in paraffin-embedded human liver tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRDX6 in paraffin-embedded human kidney tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRDX6 in paraffin-embedded human colon cancer tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PRDX6 in PC-3M cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG.