BS1890

antibody from Bioworld Technology, Inc

Targeting: SUPT3H SPT3, SPT3L

Immunohistochemistry

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Validation data

Product number: BS1890 - Provider product page
Provider: Bioworld Technology, Inc
Proper citation: Bioworld Technology Cat#BS1890, RRID:AB_1663057
Product name: SPT3 (E203) polyclonal antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide, corresponding to amino acids 181-230 of Human SPT3.
Description: The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from Ñ´ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100ul
Concentration: 1 mg/ml
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SUPT3H protein structure - BS1890 shown in red.