MA5-16199

antibody from Invitrogen Antibodies

Targeting: S100A7 PSOR1, S100A7c

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

MA5-16199

Provider

Invitrogen Antibodies

Product name

S100A7 Monoclonal Antibody (47C1068)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Suggested positive control: MCF10A, MCF-7.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

47C1068

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Psoriasin/HID-5 in A) MCF10Aand B) MCF7 cell lysateusing a Psoriasin/HID5/S100A7 monoclonal antibody (Product # MA5-16199) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of S100A7 in 0.05 mg/mL MCF-7 lysate. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of S100A7 in A) MCF10A and B) MCF7 cell lysate. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199) using a dilution of 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of S100A7 in 0.5 mg/mL MCF-7 lysate. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of S100A7 in (A) untreated and (B) LPS-treated (100 ng/mL, 21 hrs) HOPE-fixed NCI-H727 cells. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199). (C) Positive control is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 µg/mL) antibody. (D) Negative control was included omitting the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of S100A7 in Tissue section of human esophageal squamous cell carcinoma (SCC). Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199) using a dilution of 5 µg/mL. Diffused to granular cytoplasmic immunostaining was observed all over the tissue section with highest expression levels in the areas with cancer cells especially in the developing squamous keratin pearls.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of S100A7 in formalin-fixed paraffin-embedded tissue section of human lymph node cancer. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199) using a dilution of 5 µg/mL. Very strong cytoplasmic immunopositivity of Psoriasin/S100A7 protein was observed in the cancer cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of S100A7 in Human breast tissue. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199) using a dilution of 1:200 followed by a peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of S100A7 in formalin-fixed paraffin-embedded human skin tissue after heat-induced antigen retrieval. Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199) using a dilution of 10 µg/mL.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of S100A7 in Jurkat cells (blue) and a matched isotype control (orange). Samples were incubated in S100A7 monoclonal antibody (Product # MA5-16199) using a dilution of 1 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.