14951-1-AP

antibody from Invitrogen Antibodies

Targeting: KARS1 DFNB89, KARS, KARS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14951-1-AP

Provider

Invitrogen Antibodies

Product name

KARS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IRSKIITYI RSFLDELGFL EIETPMMNII PGGAVAKPFI TYHNELDMNL YMRIAPELYH KMLVVGGIDR VYEIGRQFRN EGIDLTHNPE FTTCEFYMAY ADYHDLMEIT EKMVSGMVKH ITGSYKVTYH PDGPEGQAYD VDFTPPFRRI NMVEELEKAL GMKLPETNLF ETEETRKILD DICVAKAVEC PPPRTTARLL DKLVGEFLEV TCINPTFICD HPQIMSPLAK WHRSKEGLTE RFELFVMKKE ICNAYTELND PMRQRQLFEE QAKAKAAGDD EAMFIDENFC TALEYGLPPT AGWGMGIDRV AMFLTDSNNI KEVLLFPAMK PEDKKENVAT TDTLESTTVG TSV (246-597 aa encoded by BC004132)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (Product # 14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14951-1-AP (KARS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 40x lens).