Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX30164 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30164, RRID:AB_372531
- Product name
- Progesterone Receptor (phospho Ser190) antibody [1154]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ser190-phosphorylated Progesterone Receptor in untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Progesterone Receptor (phospho Ser190) antibody [1154] at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.