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LearnNBP1-31381
antibody from Novus Biologicals
Targeting: NUP62
DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, p62, SNDI
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-31381 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31381, RRID:AB_10003796
- Product name
- Rabbit Polyclonal NUP62 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The cycloartane triterpenoid ADCX impairs autophagic degradation through Akt overactivation and promotes apoptotic cell death in multidrug-resistant HepG2/ADM cells.
Sun H, Huang M, Yao N, Hu J, Li Y, Chen L, Hu N, Ye W, Chi-Shing Tai W, Zhang D, Chen S
Biochemical pharmacology 2017 Dec 15;146:87-100
Biochemical pharmacology 2017 Dec 15;146:87-100
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - PC-12 whole cell lysate (30 ug) stained wtih Nucleoporin p62 Antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7. 5% SDS PAGE; antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nucleoporin p62 Antibody [NBP1-31381] - A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of NUP62 protein by 4 ug Nucleoporin p62 antibody 7.5 % SDS-PAGE The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG) was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nucleoporin p62 Antibody [NBP1-31381] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
